| Congresso Brasileiro de Microbiologia 2023 | Resumo: 122-1 | ||||
Resumo:Biological fouling, commonly referred to as biofouling, is a process characterized by the accumulation of interacting species, forming unique communities. Micro- and macroorganisms involved in this process substantially impact marine vessels they associate with, leading to increased fuel consumption and the propagation of invasive marine species. Biofilms, predominantly comprised of extracellular polymeric substances, are the principal culprits of biofouling in most industrial contexts. Understanding and predicting the structure of these intricate communities is vital for effective management. In the present study, we utilized a set of eight species in experimental settings to predict interactions in groups of three, derived from observations of pairwise interaction experiments. We determined community compositions via measuring culture optical density and counting colonies on solid agar media, both of which are species-specific. Our pairwise competition experiments resulted in either stable coexistence or competitive exclusion of one species. In the majority of pairings (75 out of 84, equivalent to 89.28%), one specie was observed to invade the other, whereas in the remaining pairs (11 out of 84, equivalent to 13.09%), both species could invade each other, leading to stable coexistence. Notably, we observed coexistence in two-species cocultures, irrespective of the initial concentration of each species. Subsequently, we predicted the composition of trios computationally, based on outcomes from synthetic microbial communities comprised of two species of marine bacteria. The interaction outcomes in these trios were diverse. In most cases, one unique pair survived (24 out of 56, or 42.86%). Sometimes, one species outcompeted the others (15 out of 56, or 26.78%), or a single different pair survived, with two of the three possible pairs being observed in each experiment (15 out of 56, or 26.78%). Notably, the coexistence of all three species was witnessed in a small fraction of the trios (2 out of 56, or 3.58%). Our results indicate that the initial abundance of species can shape community structure. Approaches like ours are pivotal in predicting the structure and response of communities to biofouling, providing essential insights into its management. Palavras-chave: bacterial interaction, biofouling, coexistence, exclusion, assembly rules Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e Fundação de Apoio à Pesquisa do Rio Grande do Sul (FAPERGS). | |||||